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    Dolomite creates unique microfluidics chip for creation of single cell libraries

    Dolomite, a world leader in microfluidics innovation, has launched the Single Cell RNA-Seq Chip for simple, reliable creation of single cell libraries. This new chip allows researchers to use droplet-based microfluidic methods to help explore the behaviour of thousands of individual cells, providing a rapid and easy way to screen for new or cryptic cell types.

    single cell RNA seq chip

    The Single Cell RNA-Seq Chip – a commercially available version of the prototype PDMS Drop--seq chip described by Macosko et al 1 – is manufactured from glass, offering excellent optical transparency and ensuring high precision. Combined with a very gentle sample loop method – to minimise bead shearing and contamination due to premature cell lysis – it enables rapid, straightforward preparation of single cell cDNA libraries. The chip offers robust and reproducible processing of 1 ml samples in approximately 12 hours, allowing cells to be individually encapsulated with uniquely barcoded RNA capture beads for high throughput sequencing.

    For more information, visit the Single Cell RNA-Seq Chip webpage.

    Read the Single Cell RNA-seq Chip application note

    1. Macosko, Evan Z. et al (2015). Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets. Cell, 161(5): 1202-1214.

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